The 41-year-old woman could not be resuscitated at the scene. Authorities closed the road to traffic for several hours following the incident.
The public prosecutor's office has ordered an investigation into the accident.
Emergency fire crews attended from Ermdorf, Larochette, Medernach and Waldbillig, accompanied by paramedics from Larochette.
Earlier on Thursday one person suffered serious injury following an accident between Eschdorf and Heiderscheidergrund. The motorcyclist, who had fallen from their bike, was assisted by first responders from Wiltz and Alebesch.
